| 產(chǎn)品編號 | bsm-60792R |
| 英文名稱 | CD99 Recombinant Rabbit mAb |
| 中文名稱 | CD99重組兔單克隆抗體 |
| 別 名 | T-cell surface glycoprotein E; T cell surface glycoprotein E2 precursor; 12E7; Antigen identified by monoclonal 12E7 Y homolog; Antigen identified by monoclonal antibodies 12E7 F21 and O13; CD 99; CD99 antigen; CD99 molecule; CD99X; E2 antigen; Ewing Sarc |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | D6E10 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:50-500,IHC-F=1:50-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD99 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016] Function: Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Subunit: Homodimer. Interacts with PILRB. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Natural killer cells. Similarity: Belongs to the CD99 family. SWISS: Q8VCN6 Gene ID: 673094 Database links: Entrez Gene: 4267 Human Entrez Gene: 673094 Mouse Omim: 313470 Human SwissProt: P14209 Human SwissProt: Q8VCN6 Mouse Unigene: 653349 Human Unigene: 423040 Mouse CD99（P30/32MIC2）是一種跨膜蛋白，為MIC2基因產(chǎn)物。分布于淋巴細胞、胸腺皮質(zhì)細胞、卵巢的顆粒細胞、胰島細胞和睪丸支持細胞。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD99) Monoclonal Antibody, Unconjugated (bsm-60792R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
