| 產(chǎn)品編號 | bsm-52422R |
| 英文名稱 | 5T4 Recombinant Rabbit mAb |
| 中文名稱 | 滋養(yǎng)層細胞糖蛋白5T4重組兔單抗 |
| 別 名 | 5T4; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4AG; M6P1; TPBG; TPBG_HUMAN; Trophoblast glycoprotein. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 23B1 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800,IP=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TPBG/5T4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TPBG is a 420 amino acid single-pass type I membrane protein expressed by all types of trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal putative signal sequence, a 310-residue extracellular region, a membrane anchorage domain and a 44-amino acid cytoplasmic tail with a potential phosphorylation site. The extracellular region has seven potential N-glycosylation sites and seven leucine-rich repeats, which are located in two regions separated by a hydrophilic stretch. Suggested to be involved in cell adhesion, TPBG may also be associated with tumor growth and progression. Subcellular Location: Membrane. Tissue Specificity: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. Similarity: Contains 6 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q13641 Gene ID: 7162 Database links: Entrez Gene: 7162 Human Entrez Gene: 21983 Mouse Omim: 190920 Human SwissProt: Q13641 Human SwissProt: Q9Z0L0 Mouse Unigene: 82128 Human Unigene: 20864 Mouse Unigene: 484180 Mouse Unigene: 43122 Rat |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: MCF-7, 2: JAR
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 70-80/85 kDa
Tissue: Human placenta
Section type: Formalin-fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-52422R
IP of MCF-7 cells extracts
IP ab incubation condition: bsm-52422R, 4°C
overnight, 1:100 dilution
WB primary ab incubation condition: bsm-52422R , room temperature 2h, 1:1000 dilution
Secondary ab: Anti-Rabbit IgG for IP (HRP)
Blocking buffer and concentration: 5%
NFDM/TBST
Diluting buffer and concentration: 5%
NFDM/TBST
1: 5% Input
2: IP with bsm-52422R
3: IP with Rabbit monoclonal IgG Isotype
Observed MW: 70-80/85 kDa
Exposure time: 120 s
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
