| 產(chǎn)品編號 | bsm-60686R |
| 英文名稱 | EEF1A1 Recombinant Rabbit mAb |
| 中文名稱 | 翻譯延伸因子EF-1a重組兔單抗 |
| 別 名 | Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu(EF-Tu); Eukaryotic elongation factor 1 A-1(eEF1A-1); Leukocyte receptor cluster member 7; EF1A1_HUMAN; EEF1A; EF1A; LENG7; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R8F9 |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應用 | WB=1:500-2000,ICC/IF=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eEF1a1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle SWISS: P68104 Gene ID: 1915 Database links:
Entrez Gene: 1915 Human Entrez Gene: 13627 Mouse SwissProt: P68104 Human SwissProt: P10126 Mouse
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| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: MCF7, 2: PANC-1, 3: Neuro-2a, 4:
Rat kidney
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 50 kDa
Observed MW: 55 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
