| 產(chǎn)品編號 | bsm-60618R |
| 英文名稱 | SATB1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H6D4 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,Flow-Cyt=1:20-1:100,ICC/IF=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 促成 Xist RNA 介導(dǎo)的 X 失活啟動的關(guān)鍵沉默因子，發(fā)生在胚胎發(fā)生和淋巴瘤中。在富含 AT 的特殊序列（共有 SATB1 結(jié)合序列 (CSBS)，在核基質(zhì)或支架相關(guān)區(qū)域）與 DNA 結(jié)合。認為識別雙鏈 DNA 的糖磷酸結(jié)構(gòu)。通過與 DNA 的基質(zhì)附著區(qū) (MAR) 結(jié)合并誘導(dǎo)局部染色質(zhì)環(huán)重塑，以磷酸化和乙?；癄顟B(tài)依賴性方式控制核和病毒基因表達的轉(zhuǎn)錄抑制因子。作為幾種染色質(zhì)重塑酶（例如 MHC-I 位點的 PML）的?？课稽c，還可以直接向啟動子和增強子募集輔抑制因子 (HDAC) 或輔激活因子 (HAT)。調(diào)節(jié)對來自胸腺細胞的免疫 T 細胞 CD8SP 的成熟至關(guān)重要的基因。需要轉(zhuǎn)換胎兒珠蛋白種類，以及在紅細胞分化過程中調(diào)節(jié) β 和 γ 珠蛋白基因。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Jurkat, 2: THP-1
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 86 kDa
Observed MW: 100 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60618R
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
