| 產(chǎn)品編號 | bsm-60237R |
| 英文名稱 | CD43 Recombinant Rabbit mAb |
| 中文名稱 | CD43重組兔單抗 |
| 別 名 | LEUK_HUMAN; Leukosialin; SPN; GPL115; GALGP; GPL 115; Galactoglycoprotein(GALGP); Leukocyte sialoglycoprotein; Sialophorin; CD43 cytoplasmic tail; CD 43; CD43 antigen; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | H6G11 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:10000-1:20000,Flow-Cyt=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 0.5mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017] Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. Database links: Entrez Gene: 6693 Human Entrez Gene: 20737 Mouse SwissProt: P16150 Human SwissProt: P15702 Mouse CD43又名白細胞唾液酸糖蛋白或唾液酸蛋白，是一種跨膜表面蛋白，在人體中有SPN（唾液蛋白）基因編碼。CD43分子中的缺陷與Wiskott-Aldrich綜合征的發(fā)病有關(guān)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human U937 cell lysates
Lane 2: Human K562 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-CD43 (bsm-60237R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 105 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
