| 產(chǎn)品編號(hào) | bs-20661R |
| 英文名稱 | NKG2D Rabbit pAb |
| 中文名稱 | NK細(xì)胞受體2D（CD314）抗體 |
| 別 名 | KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD 314; CD314 antigen; D12S2489E; Killer cell lectin like receptor subfamily K member 1; Killer cell lectin-like receptor subfamily K member 1; KLR; Klrk1; NKG2 D activating NK receptor; NKG2 D type II integral membrane protein; NKG2-D-activating NK receptor; NKG2D_HUMAN; NKLLR; NKR P2; Nkrp2. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse NKG2D: 1-100/232 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010] Function: Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. Subunit: Homodimer. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). Similarity: Contains 1 C-type lectin domain. SWISS: O54709 Gene ID: 27007 Database links: Entrez Gene: 22914 Human Entrez Gene: 27007 Mouse Omim: 611817 Human SwissProt: P26718 Human SwissProt: O54709 Mouse Unigene: 387787 Human Unigene: 8217 Mouse Unigene: 14544 Rat
NKG2-D出現(xiàn)在免疫細(xì)胞上表達(dá)的受體，NKG2D屬C型凝集素家族跨膜蛋白，廣泛表達(dá)在NK細(xì)胞、CD8+的αβT細(xì)胞和γδT細(xì)胞表面，可以提高NK細(xì)胞對(duì)腫瘤細(xì)胞的殺傷活性。 |
| 產(chǎn)品圖片 |
Sample:
Lung Cell (Mouse) Lysate at 40 ug
Primary: Anti-NKG2D (bs-20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (bs-20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (bs-20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃)).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-20661R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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