| 產(chǎn)品編號 | bs-19195R |
| 英文名稱 | NEK11 Rabbit pAb |
| 中文名稱 | 絲氨酸/蘇氨酸蛋白激酶Nek11抗體 |
| 別 名 | 4932416N14Rik; FLJ23495; NEK11; NEK11_HUMAN; NEK11S/L; Never in mitosis A-related kinase 11; NIMA(never in mitosis gene a) related kinase 11; NIMA(never in mitosis gene a)-related kinase 11; NIMA related kinase 11L; NIMA related kinase 11S; NIMA-related kinase 11; NimA-related protein kinase 11; Serine/threonine-protein kinase Nek11. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 and 74 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEK11: 151-250/645 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] Function: Possible role in S-phase checkpoint mechanism. Subcellular Location: Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. Tissue Specificity: Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Post-translational modifications: Phosphorylated. Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain. SWISS: Q8NG66 Gene ID: 79858 Database links: Entrez Gene: 79858 Human Entrez Gene: 208583 Mouse Omim: 609779 Human SwissProt: Q8NG66 Human SwissProt: Q8C0Q4 Mouse Unigene: 657336 Human Unigene: 347509 Mouse |
| 產(chǎn)品圖片 |
Sample: Hela (Human)Cell Lysate at 40 ug
Primary: Anti- NEK11(bs-19195R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 54'74kD
Observed band size: 70kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK11) Polyclonal Antibody, Unconjugated (bs-19195R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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