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HIPK1 Rabbit pAb (bs-17403R)  
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產(chǎn)品編號 bs-17403R
英文名稱 HIPK1 Rabbit pAb
中文名稱 核小體相關(guān)激酶HIPK1抗體
別    名 Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interacting protein kinase 1; Homeodomain interacting protein kinase 1 like protein; Homeodomain-interacting protein kinase 1; Myak; Nbak 2; Nbak2; Nuclear body associated kinase 2; Nuclear body associated kinase 2b.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導(dǎo)  激酶和磷酸酶  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse, Macaque Monkey)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 131 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HIPK1: 1-100/1210 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]

Function:
May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.

Subcellular Location:
Nucleus. Cytoplasm. Predominantly nuclear.

Tissue Specificity:
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.

Post-translational modifications:
Autophosphorylated. Phosphorylated and activated by JNK1.

Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain.

SWISS:
Q86Z02

Gene ID:
204851

Database links:

Entrez Gene: 204851 Human

Entrez Gene: 15257 Mouse

Entrez Gene: 365895 Rat

Omim: 608003 Human

SwissProt: Q86Z02 Human

SwissProt: O88904 Mouse

SwissProt: A4L9P5 Rat

Unigene: 532363 Human

Unigene: 20827 Mouse

Unigene: 4151 Rat



產(chǎn)品圖片
Sample: Mcf-7(Human) Cell Lysate at 40 ug Primary: Anti-HIPK1(bs-17403R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131kD
Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human HeLa cell lysates Lane 4: Human 293T cell lysates Primary: Anti-HIPK1 (bs-17403R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kDa Observed band size: 130 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (bs-17403R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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