| 產(chǎn)品編號 | bs-13924R |
| 英文名稱 | CHRAC1 Rabbit pAb |
| 中文名稱 | CHRAC1蛋白抗體 |
| 別 名 | CHARC1; CHARC15; CHRAC-1; CHRAC-15; Chrac1; CHRAC15; CHRC1_HUMAN; Chromatin accessibility complex 1; Chromatin accessibility complex 15 kDa protein; Chromatin accessibility complex protein 1; DNA polymerase epsilon subunit p15; histone-fold protein CHRAC15; HuCHRAC15; YCL1. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Sheep,Cow,Chicken) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CHRAC1: 1-100/131 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators (1). CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner (2). Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing (3). This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array (4). In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure (5). Function: Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SWISS: Q9NRG0 Gene ID: 54108 Database links: Entrez Gene: 54108 Human Omim: 607268 Human SwissProt: Q9NRG0 Human Unigene: 279704 Human |
| 產(chǎn)品圖片 |
Sample: muscle (Mouse) Lysate at 40 ug
Primary: Anti-CHRAC1(bs-13924R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kD
Observed band size: 13 kD
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-CHRAC1 (bs-13924R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 11 kDa
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