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CSNK1G2 Rabbit pAb (bs-12425R)  
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產(chǎn)品編號(hào) bs-12425R
英文名稱 CSNK1G2 Rabbit pAb
中文名稱 酪蛋白激酶1γ2/casein kinase Iγ1抗體
別    名 Casein kinase I isoform gamma-2; EC:2.7.11.1; CKI-gamma 2; CK1G2; KC1G2_HUMAN;  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Mouse (predicted: Human,Rat,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK1g2/Casein Kinase 1 gamma 2: 201-300/415 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Enables protein serine/threonine kinase activity. Involved in peptidyl-serine phosphorylation. Located in membrane. [provided by Alliance of Genome Resources, Apr 2022]

Function:
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.

Subunit:
Monomer (By similarity). Interacts with MTA1 (short isoform) in the cytoplasm. Binds to PER1 and triggers its proteasomal degradation. Interacts with SMAD3.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Testis.

Post-translational modifications:
Autophosphorylated (By similarity).

Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain.

SWISS:
P78368

Gene ID:
1455

Database links:

Entrez Gene: 1455 Human

Entrez Gene: 103236 Mouse

SwissProt: P78368 Human

SwissProt: Q8BVP5 Mouse



產(chǎn)品圖片
Blank control:Mouse kidney. Primary Antibody (green line): Rabbit Anti-Casein kinase 1 gamma 2 antibody (bs-12425R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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