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PCK1 Rabbit pAb (bs-4972R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-4972R
英文名稱 PCK1 Rabbit pAb
中文名稱 磷酸烯醇丙酮酸羧激酶抗體
別    名 PCK1; PEPC; GTP; MGC22652; PEP carboxykinase; PEPCK 1; PEPCK1; PEPCKC; phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase.  
Specific References  (5)     |     bs-4972R has been referenced in 5 publications.
[IF=6.317] Jingqi Zhao. et al. Quercetin ameliorates hepatic fat accumulation in high-fat diet-induced obese mice via PPARs. FOOD FUNCT. 2023 Jan;:  WB ;  Human.  
[IF=6.023] Li Ren. et al. Anti-inflammatory action of betulin and its potential as a dissociated glucocorticoid receptor modulator. Food Chem Toxicol. 2021 Nov;157:112539  WB ;  human.  
[IF=5.396] Yuan Liang. et al. Glucocorticoid receptor-mediated alleviation of inflammation by berberine: in vitro, in silico and in vivo investigations. Food Funct. 2021 Oct;:  WB ;  Human.  
[IF=5.279] Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Mouse.  
[IF=3.483] Qiu F et al. Metabolic effects of mulberry branch bark powder on diabetic mice based on GC-MS metabolomics approach. Nutr Metab (Lond). 2019 Jan 31;16:10.  WB ;  Mouse.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  神經(jīng)生物學(xué)  激酶和磷酸酶  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 69 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PCK1: 201-300/622 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.

Function:
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Subunit:
Monomer.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Major sites of expression are liver, kidney and adipocytes.

Post-translational modifications:
Acetylation is increased on addition of glucose and appears to regulate the protein stability.

DISEASE:
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD) [MIM:261680]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.

Similarity:
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

SWISS:
P35558

Gene ID:
5105

Database links:

Entrez Gene: 396458 Chicken

Entrez Gene: 282855 Cow

Entrez Gene: 403560 Dog

Entrez Gene: 5105 Human

Entrez Gene: 18534 Mouse

Entrez Gene: 100144531 Pig

Entrez Gene: 362282 Rat

Omim: 614168 Human

SwissProt: P05153 Chicken

SwissProt: P35558 Human

SwissProt: Q9Z2V4 Mouse

SwissProt: P07379 Rat



產(chǎn)品圖片
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-PCK1 (bs-4972R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 66 kD
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