| 產品編號 | bs-6480R |
| 英文名稱 | CH25H Rabbit pAb |
| 中文名稱 | 膽固醇25羥化酶抗體 |
| 別 名 | C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN. |
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Specific References (4) | bs-6480R has been referenced in 4 publications.
[IF=31.25] Ceglia, Simona. et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells. NAT IMMUNOL. 2023 Jan;:1-14 WB ; Mouse.
[IF=7.971] Samantha A. Hutchinson. et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. 2021 Mar;:1-12 IHC ; Human.
[IF=5.56] Mukherjee, Pallavi, et al. "Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-β and Cholesterol 25-hydroxylase in Jejunum." Journal of Lipid Research (2017): jlr-M076554. IHC-P ; Mouse.
[IF=4.122] Chattopadhyay et al. Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. (2018) Sci.Rep. 8:9032 IHC ; mouse.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CH25H: 1-100/272 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. Function: CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Subcellular Location: Endoplasmic reticulum membrane Post-translational modifications: N-glycosylated. Similarity: Belongs to the sterol desaturase family. SWISS: O95992 Gene ID: 9023 Database links: Entrez Gene: 9023 Human Entrez Gene: 12642 Mouse Omim: 604551 Human SwissProt: O95992 Human SwissProt: Q9Z0F5 Mouse |
| 產品圖片 |
Sample:
Lane 1: Human U-87 MG cell Lysates
Primary: Anti-CH25H (bs-6480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kDa
Observed band size: 32kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
