| 產(chǎn)品編號 | bs-11013R |
| 英文名稱 | ILDR1 Rabbit pAb |
| 中文名稱 | 免疫球蛋白樣結(jié)構(gòu)域受體1抗體 |
| 別 名 | ILDR1alpha; ILDR1beta; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1 alpha; Immunoglobulin like domain containing receptor 1; Immunoglobulin like domain containing receptor 1 beta; MGC50831; ILDR1_HUMAN. |
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Specific References (1) | bs-11013R has been referenced in 1 publications.
[IF=6.6] Franziska Wei?. et al. MarvelD3 Is Upregulated in Ulcerative Colitis and Has Attenuating Effects during Colitis Indirectly Stabilizing the Intestinal Barrier. CELLS-BASEL. 2022 Jan;11(9):1541 WB ; Human.
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| 研究領(lǐng)域 | 細胞生物 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ILDR1: 101-200/546 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ILDR1 is a putative membrane receptor. It belongs to the immunoglobulin superfamily, LISCH7 family. It is mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver. Function: Putative membrane receptor. Subunit: Homooligomer. Subcellular Location: Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol). Tissue Specificity: Mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver. DISEASE: Defects in ILDR1 are the cause of deafness autosomal recessive type 42 (DFNB42) [MIM:609646]; also called non-syndromic sensorineural deafness autosomal recessive type 42. DFNB42 is a prelingual, non-progressive form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Similarity: Belongs to the immunoglobulin superfamily. LISCH7 family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q86SU0 Gene ID: 286676 Database links: Entrez Gene: 286676 Human Entrez Gene: 106347 Mouse Omim: 609739 Human SwissProt: Q86SU0 Human SwissProt: Q8CBR1 Mouse Unigene: 98484 Human Unigene: 17807 Mouse |
| 產(chǎn)品圖片 |
Sample:
Prostate(Rat) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
Pancreas(Mouse) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (bs-11013R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
