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HOPX/LAGY Rabbit pAb (bs-9462R)  
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產(chǎn)品編號 bs-9462R
英文名稱 HOPX/LAGY Rabbit pAb
中文名稱 肺癌相關蛋白Y抗體
別    名 SMAP31; CAMEO; HOD; Homeodomain-only protein; HOP; HOP homeobox; HOP_HUMAN; HOPX; LAGY; Lung cancer-associated Y protein; NECC1; Not expressed in choriocarcinoma clone 1; Not expressed in choriocarcinoma protein 1; Odd homeobox protein 1; TOTO.  
研究領域 腫瘤  心血管  轉錄調(diào)節(jié)因子  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 8 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HOPX/LAGY: 21-60/73 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.

Function:
Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.

Subunit:
Interacts with serum response factor (SRF). Component of a large complex containing histone deacetylases such as HDAC2 (By similarity).

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.

Similarity:
Contains 1 homeobox DNA-binding domain.

SWISS:
Q9BPY8

Gene ID:
84525

Database links:

Entrez Gene: 281270 Cow

Entrez Gene: 84525 Human

Entrez Gene: 74318 Mouse

Entrez Gene: 396692 Pig

Entrez Gene: 171160 Rat

Omim: 607275 Human

SwissProt: Q8MJD5 Cow

SwissProt: Q9BPY8 Human

SwissProt: Q8R1H0 Mouse

SwissProt: Q8MJD6 Pig

SwissProt: Q78ZR5 Rat

Unigene: 619396 Human

Unigene: 181852 Mouse

Unigene: 478741 Mouse

Unigene: 2989 Rat




產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOPX) Polyclonal Antibody, Unconjugated (bs-9462R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti- LAGY/AF488 antibody(bs-9462R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
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