| 產(chǎn)品編號(hào) | bs-6359R |
| 英文名稱 | Glutamine PRPP amidotransferase Rabbit pAb |
| 中文名稱 | 谷氨酰胺磷酸核糖基焦磷酸酰胺基轉(zhuǎn)移酶 |
| 別 名 | Amidophosphoribosyltransferase [Precursor]; ATASE; Glutamine phosphoribosylpyrophosphate amidotransferase; Glutamine PRPP amidotransferase; GPAT; phosphoribosyl pyrophosphate amidotransferase; PPAT; PRAT; PUR1_HUMAN. |
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Specific References (1) | bs-6359R has been referenced in 1 publications.
[IF=6.59] Pengfei Zhang. et al. Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia. FRONT NUTR. 2022 Dec 19;9:1045805 WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glutamine PRPP amidotransferase: 351-430/517 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Phosphoribosyl pyrophosphate amidotransferase (PPAT) is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. The genes for PPAT and PAICS/AIRC, (a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway), are located in close proximity on chromosome 4. Subunit: Homotetramer. Tissue Specificity: Ubiquitously expressed. Similarity: n the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain. SWISS: Q06203 Gene ID: 5471 Database links: Entrez Gene: 5471 Human Entrez Gene: 231327 Mouse Omim: 172450 Human SwissProt: Q06203 Human Unigene: 331420 Human Unigene: 202337 Mouse Unigene: 18690 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamine PRPP amidotransferase Polyclonal Antibody, Unconjugated(bs-6359R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamine PRPP amidotransferase Polyclonal Antibody, Unconjugated(bs-6359R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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