| 產(chǎn)品編號 | bs-6686R |
| 英文名稱 | ICAM5 Rabbit pAb |
| 中文名稱 | 細(xì)胞間粘附分子5抗體 |
| 別 名 | ICAM5_HUMAN; ICAM5 precursor; Intercellular adhesion molecule 5; Intercellular adhesion molecule 5 precursor; Intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCN; TLN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞粘附分子 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=0.2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 97 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ICAM5: 501-600/924 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases. Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed on neurons in the most rostral segment of the mammalian brain, the telencephalon. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. Contains 9 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q9UMF0 Gene ID: 7087 Database links: Entrez Gene: 7087 Human Entrez Gene: 15898 Mouse Omim: 601852 Human SwissProt: Q9UMF0 Human SwissProt: Q60625 Mouse Unigene: 465862 Human Unigene: 59765 Rat |
| 產(chǎn)品圖片 |
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- ICAM5 (bs-6686R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 167 kD
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-ICAM5 antibody (bs-6686R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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