| 產(chǎn)品編號 | bs-6668R |
| 英文名稱 | HOXB5 Rabbit pAb |
| 中文名稱 | 同源盒蛋白HOXB5抗體 |
| 別 名 | Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox B5; Homeobox protein HHO.C10; Homeobox protein Hox B5; Homeobox protein Hox-2A; Homeobox protein Hox-B5; Homeobox protein HoxB5; Homeobox protein Hu-1; Homeobox2A; HomeoboxB5; HOX 2; Hox 2.1; HOX 2A; HOX B5; HOX2; Hox2.1; HOX2A; HOXB 5; Hoxb5; HU 1; HU1; HXB5_HUMAN. |
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Specific References (1) | bs-6668R has been referenced in 1 publications.
[IF=2.466] Chengran Xu. et al. Increased Expression of Homeobox 5 Predicts Poor Prognosis: A Potential Prognostic Biomarker for Glioma. INT J GEN MED. 2022; 15: 4399–4407 WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HOXB5: 181-269/269 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue. [provided by RefSeq, Jul 2008]. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Subcellular Location: Nucleus. Tissue Specificity: Spinal cord. Similarity: Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P09067 Gene ID: 3215 Database links: Entrez Gene: 3215 Human Entrez Gene: 15413 Mouse Omim: 142960 Human SwissProt: P09067 Human SwissProt: P09079 Mouse Unigene: 654456 Human Unigene: 207 Mouse Unigene: 229521 Rat |
| 產(chǎn)品圖片 |
Sample:
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-HOXB5? (bs-6668R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-HOXB5 antibody (bs-6668R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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