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PCMT1 Rabbit pAb (bs-6211R)  
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產(chǎn)品編號(hào) bs-6211R
英文名稱 PCMT1 Rabbit pAb
中文名稱 天門冬氨酸甲基轉(zhuǎn)移酶PCMT抗體
別    名 L isoaspartyl protein carboxyl methyltransferase; L isoaspartyl/D aspartyl methyltransferase; PCMT 1; PIMT; Protein beta aspartate methyltransferase; Protein L isoaspartate(D aspartate) O methyltransferase; PIMT_HUMAN; Protein L isoaspartyl/D aspartyl methyltransferase.  
研究領(lǐng)域 細(xì)胞生物  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 25 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PCMT1: 165-227/227 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 PCMT1 (Protein-L-isoaspartate (D-aspartate) O-methyltransferase) plays a role in the repair and/or degradation of damaged proteins by initiating the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. It catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues.

Function:
Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

Subunit:
Monomer.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

SWISS:
P22061

Gene ID:
5110

Database links:

Entrez Gene: 5110 Human

Entrez Gene: 25604 Rat

Omim: 176851 Human

SwissProt: P22061 Human

SwissProt: P23506 Mouse

SwissProt: P22062 Rat

Unigene: 279257 Human

Unigene: 258431 Mouse

Unigene: 86985 Rat



產(chǎn)品圖片
Sample: Hippocampus (Mouse) Lysate at 40 ug HL60(Human) Cell Lysate at 30 ug Primary: Anti- PCMT1 (bs-6211R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCMT1) Polyclonal Antibody, Unconjugated (bs-6211R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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