| 產品編號 | bs-3914R |
| 英文名稱 | AANAT Rabbit pAb |
| 中文名稱 | 芳香胺N-乙?；D移酶抗體 |
| 別 名 | SNAT_HUMAN; AANAT; Arylalkylamine N acetyltransferase; Serotonin N-acetyltransferase; SNAT. |
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Specific References (4) | bs-3914R has been referenced in 4 publications.
[IF=5.249] Zhang, WL.. et al. Expression of arylalkylamine n-acetyltransferase (AANAT) and acetylserotonin o-methyltransferase (ASMT) in the corpus luteum of pregnant sows and synthesis of melatonin in luteal cells. 2021 Nov 24 WB,IHC ; Pig.
[IF=2.923] Hongwei Duan. et al. Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep. THERIOGENOLOGY. 2022 Dec;: WB ; Sheep.
[IF=2.589] Xiao L et al. Profile?of?melatonin?and its?receptors?and?synthesizing?enzymes?in?cumulus-oocyte?complexes?of the?developing?sheep?antral?follicle-a?potential?estradiol-mediated?mechanism. Reprod Biol Endocrinol. 2019 Jan 3;17(1):1. IHC-P&WB ; Sheep.
[IF=1.858] Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 Aug;: IHC ; Sheep.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AANAT: 141-207/207 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Function: Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. Subunit: Monomer (By similarity). Interacts with several 14-3-3 proteins, including YWHAB, YWHAE, YWHAG and YWHAZ, preferentially when phosphorylated at Thr-31. Phosphorylation on Ser-205 also allows binding to YWHAZ, but with lower affinity. The interaction with YWHAZ considerably increases affinity for arylalkylamines and acetyl-CoA and protects the enzyme from dephosphorylation and proteasomal degradation (By similarity). It may also prevent thiol-dependent inactivation (By similarity). Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland. Post-translational modifications: cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins. DISEASE: Delayed sleep phase syndrome (DSPS) [MIM:614163]: A circadian rhythm sleep disorder characterized by sleep-onset insomnia and difficulty in awakening at the desired time. Patients with DSPS have chronic difficulty in adjusting their sleep-onset and wake-up times to occupational, school, and social activities. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry. Susceptibility to delayed sleep phase syndrome can be conferred by variant Thr-129. Thr-129 shows a significantly higher frequency in affected individuals than in healthy controls. Similarity: Belongs to the acetyltransferase family. AANAT subfamily. Contains 1 N-acetyltransferase domain. SWISS: Q16613 Gene ID: 15 Database links: Entrez Gene: 503504 Chimpanzee Entrez Gene: 15 Human Entrez Gene: 11298 Mouse SwissProt: P79774 Chicken SwissProt: Q5IS55 Chimpanzee SwissProt: Q16613 Human SwissProt: O88816 Mouse Unigene: 431417 Human Unigene: 418559 Mouse Unigene: 42233 Mouse Unigene: 88180 Rat AANAT-褪黑素合成限速酶. |
| 產品圖片 |
Sample:
Lane 1: Mouse Ovary tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Primary: Anti-AANAT (bs-3914R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AANAT) Polyclonal Antibody, Unconjugated (bs-3914R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AANAT) Polyclonal Antibody, Unconjugated (bs-3914R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
