| 產品編號 | bs-3462R |
| 英文名稱 | Phospho-Tyrosine Hydroxylase (Ser40) Rabbit pAb |
| 中文名稱 | 磷酸化酪氨酸羥化酶抗體 |
| 別 名 | Tyrosine Hydroxylase(phospho-Ser40); Tyrosine Hydroxylase; DYT14; DYT5b; ple; Protein Pale; TH; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; TY3H_HUMAN. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 神經生物學 信號轉導 轉錄調節(jié)因子 激酶和磷酸酶 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser40: RQ(p-S)LI |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease. Function: Plays an important role in the physiology of adrenergic neurons. Tissue Specificity: Mainly expressed in the brain and adrenal glands. DISEASE: Segawa syndrome autosomal recessive (ARSEGS) [MIM:605407]: A form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=The disease is caused by mutations affecting the gene represented in this entry. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. SWISS: P04177 Gene ID: 25085 Database links: Entrez Gene: 7054 Human Entrez Gene: 21823 Mouse Omim: 191290 Human SwissProt: P07101 Human SwissProt: P24529 Mouse Unigene: 435609 Human Unigene: 1292 Mouse Unigene: 11082 Rat 神經細胞標志物 酪氨酸羥化酶（TH）是兒茶酚胺類神經遞質即多巴胺、去甲腎上腺素、腎上腺素生物合成過程所需的限速酶，它以四氫生物喋呤啶(BH4)為輔酶，催化酪氨酸的羥化而生成多巴(DOPA)。 已知在患帕金森病(Parkinson disease,PD)時，腦內多巴胺(dopamine,DA)的減少與此酶活性低下有關。因此對PD模型動物來說，若將TH基因植入腦內，便可以提高腦內DA水平而達到基因治療目的。 |
| 產品圖片 |
Sample: U251 Cell lysate at 30ug;
Primary: Anti-Phospho-Tyrosine Hydroxylase (Ser40) (bs-3462R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 60 kD
Observed band size: 60 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
