| 產(chǎn)品編號 | bs-1535R |
| 英文名稱 | Alkaline Phosphatase, Tissue Non-Specific isozyme Rabbit pAb |
| 中文名稱 | 堿性磷酸酶(組織非特異性)抗體 |
| 別 名 | AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. 堿性磷酸酶，組織非特異性同工酶; 組織非特異性堿性磷酸酶; 堿性磷酸酶,組織非特異性; |
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Specific References (4) | bs-1535R has been referenced in 4 publications.
[IF=5.595] Cao D et al. Hematopoietic?stem?cells?and?lineage?cells?undergo?dynamic?alterations?under?microgravity?and?recovery?conditions.FASEB J. 2019 Feb 27:fj201802421RR. FCM ; Mouse.
[IF=4.545] Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235. IHC/IF ; Mouse.
[IF=2.147] Huijuan Shen. et al. microRNA-146a mediates distraction osteogenesis via bone mesenchymal stem cell inflammatory response. ACTA HISTOCHEM. 2022 Aug;124:151913 WB ; Dog.
[IF=1.931] Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034 WB ; Human.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Alkaline Phosphatase, Tissue Non-Specific isozyme: 52-150/524 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Function: This isozyme may play a role in skeletal mineralization. Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Post-translational modifications: Glycosylated. DISEASE: Defects in ALPL are a cause of hypophosphatasia (HOPS) [MIM:146300]. HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC) [MIM:241510]. Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI) [MIM:241500]. Similarity: Belongs to the alkaline phosphatase family. SWISS: P05186 Gene ID: 249 Database links: Entrez Gene: 249 Human Entrez Gene: 11647 Mouse Omim: 171760 Human SwissProt: P05186 Human SwissProt: P09242 Mouse Unigene: 75431 Human Unigene: 288186 Mouse Unigene: 82764 Rat ALP廣泛分布于人體肝臟、骨骼、腸、腎和胎盤等組織,孕婦、骨折愈合期、骨軟化癥。佝僂病、骨細胞癌、骨質(zhì)疏松、肝膿腫、肝結(jié)核、肝硬變、白血病、甲狀腺機能亢進時，血清堿性磷酸酶亦可升高. |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Alkaline Phosphatase, Tissue Non-Specific isozyme polyclonal antibody, unconjugated (bs-1535R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
