| 產(chǎn)品編號(hào) | bs-1209R |
| 英文名稱 | GRK2 Rabbit pAb |
| 中文名稱 | G蛋白偶合受體激酶2抗體 |
| 別 名 | G-protein coupled receptor kinase 2; ADRBK 1; ADRBK1; Adrenergic beta receptor kinase 1; BARK 1; BARK; BARK1; beta adrenergic receptor kinase 1; beta ARK 1; beta ARK1; G Protein Coupled Receptor Kinase 2; G protein dependent receptor kinase 2; FLJ16718; GRK 2; ARBK1_HUMAN. |
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Specific References (1) | bs-1209R has been referenced in 1 publications.
[IF=3.7] Taguchi et al. Tonic inhibition by G protein-coupled receptor kinase 2 of Akt/endothelial nitric-oxide synthase signaling in human vascular endothelial cells under conditions of hyperglycemia with high insulin levels. (2014) J.Pharmacol.Exp.Ther. 349:199-208 IHC ; Human.
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| 研究領(lǐng)域 | 免疫學(xué) 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Cow,Chicken,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRK2: 601-689/689 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]. Function: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Subunit: Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1. Interacts with LPAR1 and LPAR2. Interacts with RALA in response to LPAR1 activation. ADRBK1 and RALA mutually inhibit each other's binding to LPAR1. Tissue Specificity: Expressed in peripheral blood leukocytes. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain. SWISS: P25098 Gene ID: 156 Database links: Entrez Gene: 156 Human Omim: 109635 Human SwissProt: P25098 Human SwissProt: Q99MK8 Mouse Unigene: 83636 Human Unigene: 254144 Mouse Unigene: 13010 Rat G蛋白偶聯(lián)受體激酶2(GRK2)是催化激動(dòng)劑誘導(dǎo)的GPCR磷酸化以及啟動(dòng)GPCR脫敏的關(guān)鍵激酶,主要用于GRK細(xì)胞膜轉(zhuǎn)位和對(duì)GRK功能的影響. |
| 產(chǎn)品圖片 |
Tissue/cell: human stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRK2/BARK1/ADRBK1 Polyclonal Antibody, Unconjugated(bs-1209R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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