| 產(chǎn)品編號 | bs-1028R |
| 英文名稱 | Cytokeratin 19 Rabbit pAb |
| 中文名稱 | 細胞角蛋白19抗體 |
| 別 名 | 40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin 19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, typ |
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Specific References (4) | bs-1028R has been referenced in 4 publications.
[IF=5.116] Jian Liu. et al. Involvement of miRNA203 in the proliferation of epidermal stem cells during the process of DM chronic wound healing through Wnt signal pathways. Stem Cell Res Ther. 2020 Dec;11(1):1-10 IHC ; Rat.
[IF=3.267] Joung-Hee Kim. et al. Mealworm Oil (MWO) Enhances Wound Healing Potential through the Activation of Fibroblast and Endothelial Cells. Molecules. 2021 Jan;26(4):779 IHC ; Rat.
[IF=2.24] Yang Rong-Hua, et al. An improved method for the isolation and culture of rat epidermal stem cells. International Journal of Clinical and?Experimental Pathology6.11 (2013): 2529-2534. ICC ; Rat.
[IF=0] Yunsheng Wang. et al. A rare case of giant panda cancer: Pancreatic ductal adenocarcinoma. Animal Models and Experimental Medicine. 2022 Nov;: IHC ; Panda.
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| 研究領域 | 腫瘤 細胞生物 干細胞 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CK19: 168-270/400 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] Function: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Subunit: Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein. Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. Similarity: Belongs to the intermediate filament family. SWISS: P08727 Gene ID: 3880 Database links: Entrez Gene: 3880 Human Entrez Gene: 16669 Mouse Omim: 148020 Human SwissProt: P08727 Human SwissProt: P19001 Mouse Unigene: 654568 Human Unigene: 439699 Mouse 結構蛋白（Structural Proteins） CK-19細胞角蛋白-19(又稱CYFRa21-1)主要用于腫瘤方面的研究，近年來大多用于消化系統(tǒng)惡性腫瘤的鑒別，有學者認為：CK-19在胰腺干細胞分化過程中有細胞角蛋白19的表達，把細胞角蛋白19作為胰腺干細胞的標志物。陽性部位：主要在胞漿。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Cytokeratin 19 (bs-1028R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Blank control（black line）:MCF-7.
Primary Antibody (green line): Rabbit Anti-Cytokeratin 19 antibody (bs-1028R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
